The faithful execution of biological processes requires a precise and carefully orchestrated
set of steps that depend on the proper spatial and temporal expression of genes. Here we …

The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II comprises
multiple tandem conserved heptapeptide repeats, unique to this eukaryotic RNA …

Peroxisome proliferator‐activated receptor (PPAR) α, β (also known as δ), and γ function as
sensors for fatty acids and fatty acid derivatives and control important metabolic pathways …

Directed evolution experiments are typically carried out using in vitro systems, bacteria, or
yeast—even when the goal is to probe or modulate mammalian biology. Performing directed …

Throughout the genome, unstable tandem nucleotide repeats can expand to cause a variety
of neurologic disorders. Expansion of a CAG triplet repeat within a coding exon gives rise to …

Mediator is an evolutionarily conserved coregulator of RNA polymerase II transcription.
Microarray structure-function analysis of S. cerevisiae Mediator reveals functional …

The Wingless/Wnt pathway controls cell fates during animal development and regulates
tissue homeostasis as well as stem cell number and differentiation in epithelia. Deregulation …

Abstract The Mediator (Med) complex relays regulatory information from DNA-bound
transcription factors to the RNA polymerase II in eukaryotes. This macromolecular unit is …

Mass spectrometry has evolved at an exponential rate over the last 100 years. Innovations in
the development of mass spectrometers have created powerful instruments capable of …

Proteomic analysis of digested complex protein mixtures has become a useful strategy to
identify proteins involved in biological processes. We have evaluated the use of a new mass …