Water solubility and structural stability are key merits for proteins defined by the primary
sequence and 3D-conformation. Their manipulation represents important aspects of the …

Tens of millions of contrast-enhanced magnetic resonance imaging (MRI) exams are
performed annually around the world. The contrast agents, which improve diagnostic …

Bidirectional interfacing with the nervous system enables neuroscience research, diagnosis,
and therapy. This two-way communication allows us to monitor the state of the brain and its …

P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been
extensively studied over a period of almost forty years. The enzyme has been redesigned to …

Knowing how protein sequence maps to function (the “fitness landscape”) is critical for
understanding protein evolution as well as for engineering proteins with new and useful …

The development of catalytic systems for the controlled oxidation of C–H bonds remains a
highly sought-after goal in chemistry owing to the great utility of such transformation toward …

In vivo biosensors are emerging as powerful tools in biomedical research and diagnostic
medicine. Distinct from “labels” or “imaging”, in vivo biosensors are designed for continuous …

Simultaneously measuring the activities of all neurons in a mammalian brain at millisecond
resolution is a challenge beyond the limits of existing techniques in neuroscience. Entirely …

Protein engineering of cytochrome P450 monooxygenases (P450s) has been very
successful in generating valuable non-natural activities and properties, allowing these …

This review focuses on MRI contrast agents that are responsive to a change in a
physiological biomarker. The response mechanisms are dependent on six physicochemical …