Protein pharmaceuticals have become a significant class of marketed drug products and are
expected to grow steadily over the next decade. Development of a commercial protein …

Protein PEGylation represents a significant technological advancement in the development
of protein-based therapeutics and is widely used to reduce immunogenicity, enhance …

Covalent attachment of therapeutic proteins to polyethylene glycol (PEG) is widely used for
the improvement of its pharmacokinetic and pharmacological properties, as well as the …

The drying of biomolecules into powdered formulations has become the main form of long-
term product stabilisation, allowing for the delivery of safe and efficient medicines. Stability of …

Objective (s): Today, the non-covalent PEGylation methods of protein pharmaceuticals
attract more attention and possess several advantages over the covalent approach. In the …

Aggregate formation is a major problem affecting both safety and efficacy of
biopharmaceuticals and is associated with protein immunogenicity. Size exclusion high …

Hydrogels show great potential particularly in the development of drug delivery because of
their desirable properties. The enzymatic crosslinked casein hydrogel is a new generation …

The antigenicity of β-lactoglobulin (β-LG) can be influenced by pH values and reduced by
epigallocatechin-3-gallate (EGCG). However, a detailed mechanism concerning EGCG …

Abstract β-lactoglobulin (β-LG) is one of the main allergens in milk. Polyethylene glycol
(PEG) modification (PEGylation) was found to have the ability to reduce the antigenicity of …

In this study, through a combined simulated enzymolysis-molecular docking-molecular
simulation-activity determination-action mechanism strategy, we screened a β-LG-derived …