Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Transcription-factor-based biosensors (TFBs) are often used for metabolite detection,
adaptive evolution, and metabolic flux control. However, designing TFBs with superior …

The production efficiency of microbial cell factories is sometimes limited by the lack of
effective methods to regulate multiple targets in a coordinated manner. Here taking the …

Enzymes that can decompose synthetic plastics such as polyethylene terephthalate (PET)
are urgently needed. Still, a bottleneck remains due to a lack of techniques for detecting and …

Directed evolution has become one of the most successful and powerful tools for protein
engineering. However, the efforts required for designing, constructing, and screening a large …

Background Advanced DNA synthesis, biosensor assembly, and genetic circuit
development in synthetic biology and metabolic engineering have reinforced the application …

Fluorescence-activated droplet sorting (FADS) has emerged as a powerful tool for ultrahigh-
throughput screening of enzymes, metabolites, and antibodies. Fluorescence coupling …

The remarkable metabolic diversity observed in nature has provided a foundation for
sustainable production of a wide array of valuable molecules. However, transferring the …

A variety of chemicals have been produced through metabolic engineering approaches, and
enhancing biosynthesis performance can be achieved by using enzymes with high catalytic …

Filamentous fungi are competitive hosts for the production of drugs, proteins, and chemicals.
However, their utility is limited by screening methods and low throughput. In this work, a …