Cells have the ability to respond to various types of environmental cues, and in many cases
these cues induce directed cell migration towards or away from these signals. How cells …

Actin dynamics in cell motility, division, and phagocytosis is regulated by complex factors
with multiple feedback loops, often leading to emergent dynamic patterns in the form of …

The RAS-Extracellular signal-regulated kinase (RAS-ERK) pathway plays a conserved role
in promoting cell migration and invasion. Growth factors, adhesion, and oncogenes activate …

During cell migration and polarization, numerous signal transduction and cytoskeletal
components self-organize to generate localized protrusions. Although biochemical and …

Malignancy is associated with changes in cell mechanics that contribute to extensive cell
deformation required for metastatic dissemination. We hypothesized that the cell-intrinsic …

Ras proteins are essential mediators of a multitude of cellular processes, and its
deregulation is frequently associated with cancer appearance, progression, and metastasis …

Genetically encoded fluorescent biosensors are powerful tools for monitoring biochemical
activities in live cells, but their multiplexing capacity is limited by the available spectral …

Ras has been extensively studied as a promoter of cell proliferation, whereas few studies
have explored its role in migration. To investigate the direct and immediate effects of Ras …

The plasma membrane is widely regarded as the hub of the numerous signal transduction
activities. Yet, the fundamental biophysical mechanisms that spatiotemporally …

In this article, we provide detailed protocols on using optogenetic dimerizers to acutely
perturb activities of guanine nucleotide exchange factors (GEFs) specific to Ras, Rac or Rho …