A decade of advances in transposon-insertion sequencing

AK Cain, L Barquist, AL Goodman, IT Paulsen… - Nature Reviews …, 2020 - nature.com
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

The physiology and genetics of bacterial responses to antibiotic combinations

R Roemhild, T Bollenbach, DI Andersson - Nature Reviews …, 2022 - nature.com
Several promising strategies based on combining or cycling different antibiotics have been
proposed to increase efficacy and counteract resistance evolution, but we still lack a deep …

Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing

MC Lucas, LP Pryszcz, R Medina, I Milenkovic… - Nature …, 2024 - nature.com
Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been
difficult in part because a simple method to simultaneously quantify their abundance and …

APOE4 disrupts intracellular lipid homeostasis in human iPSC-derived glia

G Sienski, P Narayan, JM Bonner, N Kory… - Science translational …, 2021 - science.org
The E4 allele of the apolipoprotein E gene (APOE) has been established as a genetic risk
factor for many diseases including cardiovascular diseases and Alzheimer's disease (AD) …

Genetic compensation: A phenomenon in search of mechanisms

MA El-Brolosy, DYR Stainier - PLoS genetics, 2017 - journals.plos.org
Several recent studies in a number of model systems including zebrafish, Arabidopsis, and
mouse have revealed phenotypic differences between knockouts (ie, mutants) and …

A global genetic interaction network maps a wiring diagram of cellular function

M Costanzo, B VanderSluis, EN Koch, A Baryshnikova… - Science, 2016 - science.org
INTRODUCTION Genetic interactions occur when mutations in two or more genes combine
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …

Gene essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras

T Wang, H Yu, NW Hughes, B Liu, A Kendirli, K Klein… - Cell, 2017 - cell.com
The genetic dependencies of human cancers widely vary. Here, we catalog this
heterogeneity and use it to identify functional gene interactions and genotype-dependent …

[HTML][HTML] Functional profiling of a Plasmodium genome reveals an abundance of essential genes

E Bushell, AR Gomes, T Sanderson, B Anar, G Girling… - Cell, 2017 - cell.com
The genomes of malaria parasites contain many genes of unknown function. To assist drug
development through the identification of essential genes and pathways, we have measured …

The ecology and evolution of pangenomes

MA Brockhurst, E Harrison, JPJ Hall, T Richards… - Current Biology, 2019 - cell.com
Since the first genome-scale comparisons, it has been evident that the genomes of many
species are unbound by strict vertical descent: Large differences in gene content can occur …

The life and times of a tRNA

EM Phizicky, AK Hopper - RNA, 2023 - rnajournal.cshlp.org
The study of eukaryotic tRNA processing has given rise to an explosion of new information
and insights in the last several years. We now have unprecedented knowledge of each step …