Light-sheet microscopy: a tutorial

OE Olarte, J Andilla, EJ Gualda… - Advances in Optics and …, 2018 - opg.optica.org
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

The light-sheet microscopy revolution

JM Girkin, MT Carvalho - Journal of Optics, 2018 - iopscience.iop.org
This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …

Single-frame deep-learning super-resolution microscopy for intracellular dynamics imaging

R Chen, X Tang, Y Zhao, Z Shen, M Zhang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy (SMLM) can be used to resolve subcellular
structures and achieve a tenfold improvement in spatial resolution compared to that …

3D single-molecule super-resolution microscopy with a tilted light sheet

AK Gustavsson, PN Petrov, MY Lee… - Nature …, 2018 - nature.com
Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a novel,
tilted light sheet illumination strategy with long axial range point spread functions (PSFs) for …

3D high-and super-resolution imaging using single-objective SPIM

R Galland, G Grenci, A Aravind, V Viasnoff, V Studer… - Nature …, 2015 - nature.com
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with
micromirrored cavities combined with a laser beam–steering unit installed on a standard …

Aberrations and adaptive optics in super-resolution microscopy

M Booth, D Andrade, D Burke, B Patton… - …, 2015 - academic.oup.com
As one of the most powerful tools in the biological investigation of cellular structures and
dynamic processes, fluorescence microscopy has undergone extraordinary developments in …

Superresolution imaging reveals nanometer-and micrometer-scale spatial distributions of T-cell receptors in lymph nodes

YS Hu, H Cang, BF Lillemeier - Proceedings of the National …, 2016 - National Acad Sciences
T cells become activated when T-cell receptors (TCRs) recognize agonist peptides bound to
major histocompatibility complex molecules on antigen-presenting cells. T-cell activation …

Super-resolution microscopy in plant cell imaging

G Komis, O Šamajová, M Ovečka, J Šamaj - Trends in plant science, 2015 - cell.com
Although the development of super-resolution microscopy methods dates back to 1994,
relevant applications in plant cell imaging only started to emerge in 2010. Since then, the …

Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution

MBM Meddens, S Liu, PS Finnegan… - Biomedical Optics …, 2016 - opg.optica.org
We have developed a method for performing light-sheet microscopy with a single high
numerical aperture lens by integrating reflective side walls into a microfluidic chip. These …

Recent progress in light sheet microscopy for biological applications

K Chatterjee, FW Pratiwi, FCM Wu, P Chen… - Applied …, 2018 - opg.optica.org
The introduction of light sheet fluorescence microscopy (LSFM) has overcome the
challenges in conventional optical microscopy. Among the recent breakthroughs in …