With both catalytic and genetic functions, ribonucleic acid (RNA) is perhaps the most
pluripotent chemical species in molecular biology, and its functions are intimately linked to …

In biology, nucleic acids are carriers of molecular information: DNA's base sequence stores
and imparts genetic instructions, while RNA's sequence plays the role of a messenger and a …

Although the mode of action of the ribosomes, the multi-component universal effective
protein-synthesis organelles, has been thoroughly explored, their mere appearance …

An improved dynamic programming algorithm is reported for RNA secondary structure
prediction by free energy minimization. Thermodynamic parameters for the stabilities of …

The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …

Phage T4 has provided countless contributions to the paradigms of genetics and
biochemistry. Its complete genome sequence of 168,903 bp encodes about 300 gene …

RNAs that contain specific high-affinity binding sites for small molecule ligands immobilized
on a solid support are present at a frequency of roughly one in 1010-1011 in pools of …

▪ Abstract The evidence showing that the self-assembly of complex RNAs occurs in discrete
transitions, each relating to the folding of sub-systems of increasing size and complexity …

Analysis of the 2.4-Å resolution crystal structure of the large ribosomal subunit from
Haloarcula marismortui reveals the existence of an abundant and ubiquitous structural motif …

Publisher Summary This chapter discusses the experimental methods needed to acquire a
melting curve and the analysis and interpretation of the data. Any standard commercial UV …