Advances in microfluidic strategies for single-cell research

Y Liu, Z Fan, L Qiao, B Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
A core challenge in biomedical and life science research is to decipher cell-to-cell
differences under pathological progression or external perturbation. Microfluidics-based …

Single-cell antigen-specific landscape of CAR T infusion product identifies determinants of CD19-positive relapse in patients with ALL

Z Bai, S Woodhouse, Z Zhao, R Arya, K Govek… - Science …, 2022 - science.org
A notable number of acute lymphoblastic leukemia (ALL) patients develop CD19-positive
relapse within 1 year after receiving chimeric antigen receptor (CAR) T cell therapy. It …

Microfluidics-based single cell analysis: from transcriptomics to spatiotemporal multi-omics

J Cao, X Chen, S Huang, W Shi, Q Fan, Y Gong… - TrAC Trends in …, 2023 - Elsevier
Uncover cell composition, cellular spatial distribution, and cell-to-cell interactions in tissues
are significant for the understanding of physiological and pathological processes. Over the …

Dielectrophoresis-assisted removal of Cd and Cu heavy metal ions by using Chlorella microalgae

K Zhao, X Zhao, T Gao, X Li, G Wang, X Pan… - Environmental …, 2023 - Elsevier
A novel dielectrophoresis (DEP)-assisted device for the bioremediation of heavy metal ions
by using Chlorella microalgae is presented in this paper. To generate the DEP forces, pairs …

Greatly enhanced CTC culture enabled by capturing CTC heterogeneity using a PEGylated PDMS–Titanium–Gold electromicrofluidic device with glutathione …

EA Kwizera, W Ou, S Lee, S Stewart, JG Shamul, J Xu… - ACS …, 2022 - ACS Publications
The circulating tumor cells (CTCs, the root cause of cancer metastasis and poor cancer
prognosis) are very difficult to culture for scale-up in vitro, which has hampered their use in …

Single-cell extracellular vesicle analysis by microfluidics and beyond

G Wu, Y Zhao, X Li, MM Ali, S Jia, Y Ren… - TrAC Trends in Analytical …, 2023 - Elsevier
Extracellular vesicles (EVs) are membrane vesicles encapsulated by lipid membranes
present in most body fluids. These EVs contain abundant biomolecular composition …

Recent advance in cell patterning techniques: Approaches, applications and future prospects

W Yang, Z Wang, T Yu, Y Chen, Z Ge - Sensors and Actuators A …, 2022 - Elsevier
Cell patterning technology is an effective technique to understand cell behaviors by
precisely controlling the position of cells revealing the major contributions of cells in basic …

Selective single-cell sorting using a multisectorial electroactive nanowell platform

L Menze, PA Duarte, L Haddon, M Chu, J Chen - ACS nano, 2021 - ACS Publications
Current approaches in targeted patient treatments often require the rapid isolation of specific
rare target cells. Stream-based dielectrophoresis (DEP) based cell sorters have the …

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

PA Duarte, L Menze, L Shoute, J Zeng… - ACS …, 2021 - ACS Publications
In this study, we present a microdevice for the capture and quantification of Sclerotinia
sclerotiorum spores, pathogenic agents of one of the most harmful infectious diseases of …

Microfluidic platform for omics analysis on single cells with diverse morphology and size: A review

S Lin, D Feng, X Han, L Li, Y Lin, H Gao - Analytica Chimica Acta, 2024 - Elsevier
Background 1 Microfluidic techniques have emerged as powerful tools in single-cell
research, facilitating the exploration of omics information from individual cells. Cell …