Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively …
Fluorescence correlation spectroscopy (FCS) is a flexible and widely used tool routinely exploited in vivo. While FCS provides estimates of dynamical quantities, such as diffusion …
For many classes of biomolecules, population-level heterogeneity is an essential aspect of biological function─ from antibodies produced by the immune system to post-translationally …
EM Patrick, JD Slivka, B Payne, MJ Comstock… - Nature chemical …, 2020 - nature.com
Telomere maintenance by telomerase is essential for continuous proliferation of human cells and is vital for the survival of stem cells and 90% of cancer cells. To compensate for …
We present a unified conceptual framework and the associated software package for single- molecule Förster resonance energy transfer (smFRET) analysis from single-photon arrivals …
Fluorescence time traces are used to report on dynamical properties of molecules. The basic unit of information in these traces is the arrival time of individual photons, which carry …
Förster resonance energy transfer (FRET) using pulsed illumination has been pivotal in leveraging lifetime information in FRET analysis. However, there remain major challenges in …
Summary Hidden Markov models (HMMs) are used to learn single-molecule kinetics across a range of experimental techniques. By their construction, HMMs assume that single …
K Liu, X Chen, CM Kaiser - Proceedings of the National …, 2019 - National Acad Sciences
Large proteins with multiple domains are thought to fold cotranslationally to minimize interdomain misfolding. Once folded, domains interact with each other through the formation …