Cell Synchronization Enhances Nuclear Transformation and Genome Editing via Cas9 Enabling Homologous Recombination in Chlamydomonas reinhardtii

M Angstenberger, F de Signori, V Vecchi… - ACS synthetic …, 2020 - ACS Publications
In Chlamydomonas reinhardtii, the model organism for eukaryotic green algae and plants,
the processes of nuclear transformation and genome editing in particular are still marked by …

Marker-free genetic manipulations in yeast using CRISPR/CAS9 system

I Soreanu, A Hendler, D Dahan, D Dovrat, A Aharoni - Current genetics, 2018 - Springer
The budding yeast is currently one of the major model organisms for the study of a wide
variety of biological processes. Genetic manipulation of yeast involves the extensive usage …

CRISPR-UnLOCK: multipurpose Cas9-based strategies for conversion of yeast libraries and strains

E Roggenkamp, RM Giersch, E Wedeman… - Frontiers in …, 2017 - frontiersin.org
Saccharomyces cerevisiae continues to serve as a powerful model system for both basic
biological research and industrial application. The development of genome-wide collections …

Transformation of Diplonema papillatum, the type species of the highly diverse and abundant marine microeukaryotes Diplonemida (Euglenozoa)

B Kaur, M Valach, P Peña‐Diaz… - Environmental …, 2018 - Wiley Online Library
Diplonema papillatum is the type species of diplonemids, which are among the most
abundant and diverse heterotrophic microeukaryotes in the world's oceans. Diplonemids are …

Fine-tuning the expression of target genes using a DDI2 promoter gene switch in budding yeast

Y Wang, K Zhang, H Li, X Xu, H Xue, P Wang… - Scientific Reports, 2019 - nature.com
Tuned gene expression is crucial to the proper growth and response to the environmental
changes of an organism. To enable tunable gene expression as designed is desirable in …

amdS as a dominant recyclable marker in Cryptococcus neoformans

PE Erpf, CJ Stephenson, JA Fraser - Fungal Genetics and Biology, 2019 - Elsevier
While the fungal pathogen Cryptoccocus neoformans is a leading cause of death in
immunocompromised individuals, the molecular toolkit currently available to study this …

Charged Polypeptide Tail Boosts the Salt Resistance of Enzyme-Containing Complex Coacervate Micelles

R Kembaren, AH Westphal, M Kamperman… - …, 2022 - ACS Publications
Encapsulation of proteins can have advantages for their protection, stability, and delivery
purposes. One of the options to encapsulate proteins is to incorporate them in complex …

Zinc finger 4 negatively controls the transcriptional activator Fzf1 in Saccharomyces cerevisiae

Y Du, C Ma, SA Moore, W Xiao - Mlife, 2024 - Wiley Online Library
Fzf1 is a Saccharomyces cerevisiae transcription factor containing five zinc fingers (ZFs). It
regulates the expression of at least five downstream genes, including SSU1, YHB1, DDI2/3 …

Utilizing biotinylated proteins expressed in yeast to visualize DNA–protein interactions at the single-molecule level

H Xue, Y Bei, Z Zhan, X Chen, X Xu, YV Fu - Frontiers in microbiology, 2017 - frontiersin.org
Much of our knowledge in conventional biochemistry has derived from bulk assays.
However, many stochastic processes and transient intermediates are hidden when …

Transcriptional activation of budding yeast DDI2/3 through chemical modifications of Fzf1

A Lin, P Chumala, Y Du, C Ma, T Wei, X Xu… - Cell Biology and …, 2023 - Springer
Abstract DDI2 and DDI3 (DDI2/3) are two identical genes in Saccharomyces cerevisiae
encoding cyanamide (CY) hydratase. They are not only highly induced by CY, but also by a …