A synthetic lethal interaction occurs between two genes when the perturbation of either gene
alone is viable but the perturbation of both genes simultaneously results in the loss of …

Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art
instrumentation or the need for years of hands-on experience, for better or worse, ensures …

Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …

Forward genetic screens are powerful tools for the discovery and functional annotation of
genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short …

Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a …

The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-
wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated …

The simplicity of programming the CRISPR (clustered regularly interspaced short
palindromic repeats)–associated nuclease Cas9 to modify specific genomic loci suggests a …

With the completion of the human genome sequence, attention turned to identifying and
annotating its functional DNA elements. As a complement to genetic and comparative …

Head and neck squamous cell carcinomas (HNSCCs) are caused by tobacco and alcohol
consumption and by infection with high-risk types of human papillomavirus (HPV). Tumours …

Evolution of large asexual cell populations underlies∼ 30% of deaths worldwide, including
those caused by bacteria, fungi, parasites, and cancer. However, the dynamics underlying …