Transferrin-mediated cellular iron delivery

AN Luck, AB Mason - Current topics in membranes, 2012 - Elsevier
Essential to iron homeostasis is the transport of iron by the bilobal protein human serum
transferrin (hTF). Each lobe (N-and C-lobe) of hTF forms a deep cleft which binds a single Fe …

Recombinant human transferrin: beyond iron binding and transport

ME Brandsma, AM Jevnikar, S Ma - Biotechnology advances, 2011 - Elsevier
Iron is indispensible for life and essential for such processes as oxygen transport, electron
transfer and DNA synthesis. Transferrin (Tf) is a ubiquitous protein with a central role in iron …

N-glycan patterns of human transferrin produced in Trichoplusia ni insect cells: effects of mammalian galactosyltransferase

E Ailor, N Takahashi, Y Tsukamoto, K Masuda… - …, 2000 - academic.oup.com
The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed
to examine N-linked oligosaccharide processing in insect cells. Metabolic radiolabeling of …

Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.)

D Zhang, S Nandi, P Bryan, S Pettit, D Nguyen… - Protein expression and …, 2010 - Elsevier
Transferrin is an essential ingredient used in cell culture media due to its crucial role in
regulating cellular iron uptake, transport, and utilization. It is also a promising drug carrier …

Insect transferrin functions as an antioxidant protein in a beetle larva

BY Kim, KS Lee, YM Choo, I Kim, YH Je… - … and Physiology Part B …, 2008 - Elsevier
In insects transferrin is known as an iron transporter, an antibiotic agent, a vitellogenin, and
a juvenile hormone regulated protein. Here, a novel functional role for insect transferrin as …

Molecular characterization of iron binding proteins, transferrin and ferritin heavy chain subunit, from the bumblebee Bombus ignitus

D Wang, BY Kim, KS Lee, HJ Yoon, Z Cui, W Lu… - … and Physiology Part B …, 2009 - Elsevier
Transferrin and ferritin are iron-binding proteins involved in transport and storage of iron as
part of iron metabolism. Here, we describe the cDNA cloning and characterization of …

Transferrin trojan horses as a rational approach for the biological delivery of therapeutic peptide domains

SA Ali, HC Joao, F Hammerschmid, J Eder… - Journal of Biological …, 1999 - ASBMB
One novel approach for the biological delivery of peptide drugs is to incorporate the
sequence of the peptide into the structure of a natural transport protein, such as human …

Expression, purification, and characterization of recombinant nonglycosylated human serum transferrin containing a C-terminal hexahistidine tag

AB Mason, QY He, TE Adams, DR Gumerov… - Protein expression and …, 2001 - Elsevier
Attachment of a hexa-His tag is a common strategy in recombinant protein production. The
use of such a tag greatly simplifies the purification of the protein from the complex mixture of …

Expression of recombinant human interferon-γ with antiviral activity in the bi-cistronic baculovirus-insect/larval system

WS Chen, OB Villaflores, TR Jinn… - Bioscience …, 2011 - academic.oup.com
A bi-cistronic baculovirus-insect/larval system containing a polyhedron promoter, an internal
ribosome entry site (IRES), and an egfp gene was developed as a cost-effective platform for …

Plant‐derived recombinant human serum transferrin demonstrates multiple functions

ME Brandsma, H Diao, X Wang… - Plant biotechnology …, 2010 - Wiley Online Library
Human serum transferrin (hTf) is the major iron‐binding protein in human plasma, having a
vital role in iron transport. Additionally, hTf has many other uses including antimicrobial …