Highlights•Lipoylation is a rare, but highly conserved lysine posttranslational modification.•
Lipoylated metabolic complexes are evolutionarily conserved from bacteria to humans.• …

Although the structure of lipoic acid and its role in bacterial metabolism were clear over 50
years ago, it is only in the past decade that the pathways of biosynthesis of this universally …

Biological microscopy would benefit from smaller alternatives to green fluorescent protein for
imaging specific proteins in living cells. Here we introduce PRIME (PRobe Incorporation …

SUMMARY Lipoic acid [(R)-5-(1, 2-dithiolan-3-yl) pentanoic acid] is an enzyme cofactor
required for intermediate metabolism in free-living cells. Lipoic acid was discovered nearly …

Methods for targeting of small molecules to cellular proteins can allow imaging with
fluorophores that are smaller, brighter, and more photostable than fluorescent proteins …

Sirtuins are an ancient family of NAD+-dependent deacylases connected with the regulation
of fundamental cellular processes including metabolic homeostasis and genome integrity …

Bioorthogonal chemistry holds great potential to generate difficult‐to‐access protein–protein
conjugate architectures. Current applications are hampered by challenging protein …

Chemical fluorophores offer tremendous size and photophysical advantages over
fluorescent proteins but are much more challenging to target to specific cellular proteins …

Three human mitochondrial diseases that directly affect lipoic acid metabolism result from
heterozygous missense and nonsense mutations in the LIAS, LIPT1, and LIPT2 genes …

Various methods have been established for the purpose of identifying and characterizing
protein–protein interactions (PPIs). This diverse toolbox provides researchers with options to …