Mass spectrometry (MS)-based proteomics has become a powerful technology to quantify
the entire complement of proteins in cells or tissues. Here, we review challenges and recent …

Biomedical research is undergoing a paradigm shift towards approaches centred on human
disease models owing to the notoriously high failure rates of the current drug development …

Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging
and deep sequencing. However, proteins are the main drivers of cellular function and in …

Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific
functional networks underlying biological processes. Here, we present a streamlined …

MS-based proteome profiling has become increasingly comprehensive and quantitative, yet
a persistent shortcoming has been the relatively large samples required to achieve an in …

Biological systems are composed of heterogeneous populations of cells that
intercommunicate to form a functional living tissue. Biological function varies greatly across …

Peptide identification in liquid chromatography-tandem mass spectrometry (LC-MS/MS)
experiments relies on computational algorithms for matching acquired MS/MS spectra …

Missing values weaken the power of label-free quantitative proteomic experiments to
uncover true quantitative differences between biological samples or experimental …

Single-cell proteomics by mass spectrometry (SCoPE-MS) is a recently introduced method
to quantify multiplexed single-cell proteomes. While this technique has generated great …

The analysis of ultralow input samples or even individual cells is essential to answering a
multitude of biomedical questions, but current proteomic workflows are limited in their …