Laboratory diagnosis of human brucellosis
P Yagupsky, P Morata… - Clinical microbiology …, 2019 - Am Soc Microbiol
The clinical presentation of brucellosis in humans is variable and unspecific, and thus,
laboratory corroboration of the diagnosis is essential for the patient's proper treatment. The …
laboratory corroboration of the diagnosis is essential for the patient's proper treatment. The …
PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
Reporting the limits of detection and quantification for environmental DNA assays
KE Klymus, CM Merkes, MJ Allison… - Environmental …, 2020 - Wiley Online Library
Abstract Background Environmental DNA (eDNA) analysis is increasingly being used to
detect the presence and relative abundance of rare species, especially invasive or imperiled …
detect the presence and relative abundance of rare species, especially invasive or imperiled …
[图书][B] Environmental DNA: For biodiversity research and monitoring
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …
High yield of RNA sequencing for targetable kinase fusions in lung adenocarcinomas with no mitogenic driver alteration detected by DNA sequencing and low tumor …
R Benayed, M Offin, K Mullaney, P Sukhadia… - Clinical Cancer …, 2019 - AACR
Purpose: Targeted next-generation sequencing of DNA has become more widely used in
the management of patients with lung adenocarcinoma; however, no clear mitogenic driver …
the management of patients with lung adenocarcinoma; however, no clear mitogenic driver …
False-positive and false-negative COVID-19 cases: respiratory prevention and management strategies, vaccination, and further perspectives
DS Mouliou, KI Gourgoulianis - Expert review of respiratory …, 2021 - Taylor & Francis
ABSTRACT Introduction: A novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-
CoV-2) was reported via nucleic acid identification in December, 2019. Accuracy of SARS …
CoV-2) was reported via nucleic acid identification in December, 2019. Accuracy of SARS …
Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?
It is well known that healthy gut microbiota is essential to promote host health and well‐
being. The intestinal microbiota of endothermic animals as well as fish are classified as …
being. The intestinal microbiota of endothermic animals as well as fish are classified as …
[HTML][HTML] How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
D Svec, A Tichopad, V Novosadova, MW Pfaffl… - Biomolecular detection …, 2015 - Elsevier
We have examined the imprecision in the estimation of PCR efficiency by means of standard
curves based on strategic experimental design with large number of technical replicates. In …
curves based on strategic experimental design with large number of technical replicates. In …
Real-time PCR detection chemistry
E Navarro, G Serrano-Heras, MJ Castaño, J Solera - Clinica chimica acta, 2015 - Elsevier
Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …
applications. This technology merges the polymerase chain reaction chemistry with the use …
Rapid bacterial colonization of low-density polyethylene microplastics in coastal sediment microcosms
Background Synthetic microplastics (≤ 5-mm fragments) are emerging environmental
contaminants that have been found to accumulate within coastal marine sediments …
contaminants that have been found to accumulate within coastal marine sediments …