The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

DNA assembly is an integral part of modern synthetic biology, as intricate genetic
engineering projects require robust molecular cloning workflows. Golden Gate assembly is a …

Assembling composite DNA modules from custom DNA parts has become routine due to
recent technological breakthroughs such as Golden Gate modular cloning. Using Golden …

Bacteria proficient at producing cellulose are an attractive synthetic biology host for the
emerging field of Engineered Living Materials (ELMs). Species from the Komagataeibacter …

Ralstonia solanacearum causes bacterial wilt disease in many plant species. Type III‐
secreted effectors (T3Es) play crucial roles in bacterial pathogenesis. However, some T3Es …

The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite
for many standard experiments for the functional analysis of genes, including the generation …

Abstract The Golden Gate cloning method enables the rapid assembly of multiple genes in
any user-defined arrangement. It utilizes type IIS restriction enzymes that cut outside of their …

Ralstonia solanacearum causes bacterial wilt disease in solanaceous crops. Identification of
avirulence type III-secreted effectors recognized by specific disease resistance proteins in …

Xanthomonas oryzae pathovars oryzae (Xoo) and oryzicola (Xoc) infect rice, causing
bacterial blight and bacterial leaf streak, respectively, which are two economically important …

Soil-dwelling Actinomycetes are a diverse and ubiquitous component of the global
microbiome but largely lack genetic tools comparable to those available in model species …