Genetic Manipulation and Transformation Methods for Aspergillus spp.

YE Son, HS Park - Mycobiology, 2021 - Taylor & Francis
Species of the genus Aspergillus have a variety of effects on humans and have been
considered industrial cell factories due to their prominent ability for manufacturing several …

Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage

J Lawrimore, TM Barry, RM Barry, AC York… - Molecular biology of …, 2017 - Am Soc Cell Biol
Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this
behavior remains unknown. To explore the mechanisms that drive DNA damage–induced …

A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as …

I Weyda, L Yang, J Vang, BK Ahring, M Lübeck… - Journal of …, 2017 - Elsevier
In recent years, versatile genetic tools have been developed and applied to a number of
filamentous fungi of industrial importance. However, the existing techniques have limitations …

Transformation of the cucurbit powdery mildew pathogen Podosphaera xanthii by Agrobacterium tumefaciens

J Martínez‐Cruz, D Romero, A de Vicente… - New …, 2017 - Wiley Online Library
The obligate biotrophic fungal pathogen Podosphaera xanthii is the main causal agent of
powdery mildew in cucurbit crops all over the world. A major limitation of molecular studies …

[HTML][HTML] Highly efficient transformation system for Malassezia furfur and Malassezia pachydermatis using Agrobacterium tumefaciens-mediated transformation

AM Celis, AM Vos, S Triana, CA Medina… - Journal of …, 2017 - Elsevier
Malassezia spp. are part of the normal human and animal mycobiota but are also associated
with a variety of dermatological diseases. The absence of a transformation system …

Nitric Oxide Negatively Regulates the Rapid Formation of Pleurotus ostreatus Primordia by Inhibiting the Mitochondrial aco Gene

L Hou, C Huang, X Wu, J Zhang, M Zhao - Journal of Fungi, 2022 - mdpi.com
Nitric oxide (NO) is as a signaling molecule that participates in the regulation of plant
development and in a number of physiological processes. However, the function and …

Assessment of the function of SUB6 in the pathogenic dermatophyte Trichophyton mentagrophytes

Y Shi, Q Niu, X Yu, X Jia, J Wang, D Lin… - Medical mycology, 2016 - academic.oup.com
Trichophyton mentagrophytes is a keratinophilic pathogenic fungus that infects both humans
and animals. Subtilisins are important for T. mentagrophytes virulence, particularly when …

[HTML][HTML] Agrobacterium-mediated transformation of Ceratocystis albifundus

M Sayari, MA Van der Nest, ET Steenkamp… - Microbiological …, 2019 - Elsevier
Functional association between genomic loci and specific biological traits remains lacking in
many fungi, including the African tree pathogen Ceratocystis albifundus. This is mainly …

An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus

G Han, Q Shao, C Li, K Zhao, L Jiang, J Fan… - Journal of …, 2018 - Springer
Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in
preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in …

Detection of Oil Palm Root Penetration by Agrobacterium-Mediated Transformed Ganoderma boninense, Expressing Green Fluorescent Protein

N Govender, MY Wong - Phytopathology, 2017 - Am Phytopath Society
A highly efficient and reproducible Agrobacterium-mediated transformation protocol for
Ganoderma boninense was developed to facilitate observation of the early stage infection of …