Cell-free gene expression: an expanded repertoire of applications

AD Silverman, AS Karim, MC Jewett - Nature Reviews Genetics, 2020 - nature.com
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Cell-free gene expression

D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Portable, on-demand biomolecular manufacturing

K Pardee, S Slomovic, PQ Nguyen, JW Lee, N Donghia… - Cell, 2016 - cell.com
Synthetic biology uses living cells as molecular foundries for the biosynthesis of drugs,
therapeutic proteins, and other commodities. However, the need for specialized equipment …

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

T Jaroentomeechai, JC Stark, A Natarajan… - Nature …, 2018 - nature.com
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

RW Martin, BJ Des Soye, YC Kwon, J Kay… - Nature …, 2018 - nature.com
Cell-free protein synthesis has emerged as a powerful approach for expanding the range of
genetically encoded chemistry into proteins. Unfortunately, efforts to site-specifically …

A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of conjugate vaccines

KF Warfel, A Williams, DA Wong, SE Sobol… - ACS synthetic …, 2022 - ACS Publications
Cell-free protein synthesis systems that can be lyophilized for long-term, non-refrigerated
storage and transportation have the potential to enable decentralized biomanufacturing …

On-demand biomanufacturing of protective conjugate vaccines

JC Stark, T Jaroentomeechai, TD Moeller… - Science …, 2021 - science.org
Conjugate vaccines are among the most effective methods for preventing bacterial
infections. However, existing manufacturing approaches limit access to conjugate vaccines …

Applications of synthetic biology in medical and pharmaceutical fields

X Yan, X Liu, C Zhao, GQ Chen - Signal transduction and targeted …, 2023 - nature.com
Synthetic biology aims to design or assemble existing bioparts or bio-components for useful
bioproperties. During the past decades, progresses have been made to build delicate …

Exploring the potential of cell-free protein synthesis for extending the abilities of biological systems

K Khambhati, G Bhattacharjee, N Gohil… - … in bioengineering and …, 2019 - frontiersin.org
Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …

Advances in cell‐free biosensors: principle, mechanism, and applications

L Zhang, W Guo, Y Lu - Biotechnology Journal, 2020 - Wiley Online Library
Synthetic biology has promoted the development of biosensors as tools for detecting trace
substances. In the past, biosensors based on synthetic biology have been designed on …