High throughput sequencing for plant virus detection and discovery

DEV Villamor, T Ho, M Al Rwahnih, RR Martin… - …, 2019 - Am Phytopath Society
Over the last decade, virologists have discovered an unprecedented number of viruses
using high throughput sequencing (HTS), which led to the advancement of our knowledge …

Recent advances on detection and characterization of fruit tree viruses using high-throughput sequencing technologies

VI Maliogka, A Minafra, P Saldarelli, AB Ruiz-García… - Viruses, 2018 - mdpi.com
Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted
through vegetative propagation and grafting of infected plant material. Some of these …

Is there a “biological desert” with the discovery of new plant viruses? A retrospective analysis for new fruit tree viruses

W Hou, S Li, S Massart - Frontiers in Microbiology, 2020 - frontiersin.org
High throughput sequencing technologies accelerated the pace of discovery and
identification of new viral species. Nevertheless, biological characterization of a new virus is …

High-throughput sequencing identifies novel viruses in nectarine: Insights to the etiology of stem-pitting disease

DEV Villamor, TA Mekuria, SS Pillai… - …, 2016 - Am Phytopath Society
Recent studies have shown the superiority of high-throughput sequencing (HTS) technology
over many standard protocols for pathogen detection. HTS was initiated on fruit tree …

Deep‐sequencing revealed Citrus bark cracking viroid (CBCVd) as a highly aggressive pathogen on hop

J Jakse, S Radisek, T Pokorn, J Matousek… - Plant …, 2015 - Wiley Online Library
Newly emerging or re‐emerging diseases are a constant and significant threat to agricultural
production, so prompt and accurate identification of the causative agents is required for …

Quality assessment and validation of high-throughput sequencing for grapevine virus diagnostics

N Soltani, KA Stevens, V Klaassen, MS Hwang… - Viruses, 2021 - mdpi.com
Development of High-Throughput Sequencing (HTS), also known as next generation
sequencing, revolutionized diagnostic research of plant viruses. HTS outperforms bioassays …

Recent advances and prospects in Prunus virology

M Rubio, P Martínez‐Gómez, A Marais… - Annals of Applied …, 2017 - Wiley Online Library
The stone fruit genus Prunus, within the family Rosaceae, comprises more than 230 species,
some of which have great importance or value as ornamental or fruit crops. Prunus are …

Characterization of new isolates of Apricot vein clearing-associated virus and of a new Prunus-infecting virus: Evidence for recombination as a driving force in …

A Marais, C Faure, E Mustafayev, T Candresse - PLoS One, 2015 - journals.plos.org
Double stranded RNAs from Prunus samples gathered from various surveys were analyzed
by a deep-sequencing approach. Contig annotations revealed the presence of a potential …

Characterization by Deep Sequencing of Prunus virus T, a Novel Tepovirus Infecting Prunus Species

A Marais, C Faure, E Mustafayev, M Barone… - …, 2015 - Am Phytopath Society
Double-stranded RNAs purified from a cherry tree collected in Italy and a plum tree collected
in Azerbaijan were submitted to deep sequencing. Contigs showing weak but significant …

High throughput sequencing reveals a novel fabavirus infecting sweet cherry

DEV Villamor, SS Pillai, KC Eastwell - Archives of Virology, 2017 - Springer
The genus Fabavirus currently consists of five species represented by viruses that infect a
wide range of hosts but none reported from temperate climate fruit trees. A virus with …