Molecular techniques to interrogate and edit the Chlamydomonas nuclear genome
RE Jinkerson, MC Jonikas - The Plant Journal, 2015 - Wiley Online Library
The success of the green alga C hlamydomonas reinhardtii as a model organism is to a
large extent due to the wide range of molecular techniques that are available for its …
large extent due to the wide range of molecular techniques that are available for its …
Synthetic biology approaches to enhance microalgal productivity
The major bottleneck in commercializing biofuels and other commodities produced by
microalgae is the high cost associated with phototrophic cultivation. Improving microalgal …
microalgae is the high cost associated with phototrophic cultivation. Improving microalgal …
An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting
biological processes in photosynthetic eukaryotes. However, its usefulness has been limited …
biological processes in photosynthetic eukaryotes. However, its usefulness has been limited …
High-throughput genotyping of green algal mutants reveals random distribution of mutagenic insertion sites and endonucleolytic cleavage of transforming DNA
A high-throughput genetic screening platform in a single-celled photosynthetic eukaryote
would be a transformative addition to the plant biology toolbox. Here, we present …
would be a transformative addition to the plant biology toolbox. Here, we present …
[HTML][HTML] High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica
C Südfeld, M Hubáček, D Figueiredo… - Metabolic …, 2021 - Elsevier
The microalga Nannochloropsis oceanica is considered a promising platform for the
sustainable production of high-value lipids and biofuel feedstocks. However, current lipid …
sustainable production of high-value lipids and biofuel feedstocks. However, current lipid …
A dual system formed by the ARC and NR molybdoenzymes mediates nitrite‐dependent NO production in Chlamydomonas
A Chamizo‐Ampudia, E Sanz‐Luque… - Plant, Cell & …, 2016 - Wiley Online Library
Nitric oxide (NO) is a relevant signal molecule involved in many plant processes. However,
the mechanisms and proteins responsible for its synthesis are scarcely known. In most …
the mechanisms and proteins responsible for its synthesis are scarcely known. In most …
Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina
Background Genetic engineering of microalgae is necessary to produce economically
feasible strains for biofuel production. Current efforts are focused on the manipulation of …
feasible strains for biofuel production. Current efforts are focused on the manipulation of …
Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase
The BBSome is a complex of seven proteins, including BBS4, that is cycled through cilia by
intraflagellar transport (IFT). Previous work has shown that the membrane-associated …
intraflagellar transport (IFT). Previous work has shown that the membrane-associated …
Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin
The assembly and maintenance of most cilia and flagella rely on intraflagellar transport
(IFT). Recent in vitro studies have suggested that, together, the calponin-homology domain …
(IFT). Recent in vitro studies have suggested that, together, the calponin-homology domain …
Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding
The transition zone (TZ) of the cilium/flagellum serves as a diffusion barrier that controls the
entry/exit of ciliary proteins. Mutations of the TZ proteins disrupt barrier function and lead to …
entry/exit of ciliary proteins. Mutations of the TZ proteins disrupt barrier function and lead to …