Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

SA Bustin - Journal of molecular endocrinology, 2000 - jme.bioscientifica.com
To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

[HTML][HTML] Multi-template polymerase chain reaction

E Kalle, M Kubista, C Rensing - Biomolecular Detection and Quantification, 2014 - Elsevier
PCR is a formidable and potent technology that serves as an indispensable tool in a wide
range of biological disciplines. However, due to the ease of use and often lack of rigorous …

A fiber-optic DNA biosensor microarray for the analysis of gene expression

JA Ferguson, TC Boles, CP Adams, DR Walt - Nature biotechnology, 1996 - nature.com
A fiber-optic biosensor array is described for the simultaneous analysis of multiple DNA
sequences. A bundle of optical fibers was assembled with each fiber carrying a different …

Recent developments in the optimization of thermostable DNA polymerases for efficient applications

AR Pavlov, NV Pavlova, SA Kozyavkin… - Trends in …, 2004 - cell.com
Thermostable DNA polymerases have a crucial role in current methods of DNA amplification
and sequencing. Major improvements in these methods have been made in recent years …

Effects of single-walled carbon nanotubes on the polymerase chain reaction

D Cui, F Tian, Y Kong, I Titushikin, H Gao - Nanotechnology, 2003 - iopscience.iop.org
The effects of single-walled carbon nanotubes on the polymerase chain reaction (PCR)
were investigated via quantitative PCR product measurements, scanning electron …

Real-time PCR in food science: introduction

D Rodríguez-Lázaro, M Hernández - Current issues in molecular biology, 2013 - mdpi.com
Food safety and quality control programmes are increasingly applied throughout the
production food chain in order to guarantee added value products as well as to minimize the …

Natural selection of the major histocompatibility complex (Mhc) in Hawaiian honeycreepers (Drepanidinae)

SI Jarvi, CL Tarr, CE Mcintosh, CT Atkinson… - Molecular …, 2004 - Wiley Online Library
The native Hawaiian honeycreepers represent a classic example of adaptive radiation and
speciation, but currently face one the highest extinction rates in the world. Although multiple …

Comparison of the 5′ nuclease activities of Taq DNA polymerase and its isolated nuclease domain

V Lyamichev, MAD Brow, VE Varvel… - Proceedings of the …, 1999 - National Acad Sciences
Many eubacterial DNA polymerases are bifunctional molecules having both polymerization
(P) and 5′ nuclease (N) activities, which are contained in separable domains. We …

PCR amplification of murine immunoglobulin germline V genes: strategies for minimization of recombination artefacts

P Zylstra, HS Rothenfluh, GF Weiller… - Immunology and cell …, 1998 - Wiley Online Library
Murine immunoglobulin germline V genes exist as multiple sequences arranged in tandem
in germline DNA. Because members of V gene families are very similar, they can be …

Snapback primer genotyping with saturating DNA dye and melting analysis

L Zhou, RJ Errigo, H Lu, MA Poritz, MT Seipp… - Clinical …, 2008 - academic.oup.com
Background: DNA hairpins have been used in molecular analysis of PCR products as self-
probing amplicons. Either physical separation or fluorescent oligonucleotides with covalent …