Enzymes are efficient and specific catalysts for many essential reactions in biotechnological
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

Motivation Studying the transport paths of ligands, solvents, or ions in transmembrane
proteins and proteins with buried binding sites is fundamental to the understanding of their …

Caver Web 1.0 is a web server for comprehensive analysis of protein tunnels and channels,
and study of the ligands' transport through these transport pathways. Caver Web is the first …

Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …

How do proteins evolve? How do changes in sequence mediate changes in protein
structure, and in turn in function? This question has multiple angles, ranging from …

The widespread trade-off between stability and activity severely limits enzyme evolution.
Although some progresses have been made to overcome this limitation, the counteraction …

Biocatalysis is based on the application of natural catalysts for new purposes, for which
enzymes were not designed. Although the first examples of biocatalysis were reported more …

Transport of ligands between buried active sites and bulk solvent is a key step in the
catalytic cycle of many enzymes. The absence of evolutionary optimized transport tunnels is …

This mini review gives an overview over different design approaches and methodologies
applied in rational and semirational enzyme engineering. The underlying principles for …