Background Since the introduction of what became today's standard for cryo-embedding of
biological macromolecules at native conditions more than 30 years ago, techniques and …

Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in
recent years. It provides structures of macromolecular complexes in situ and in cellular …

Macromolecular complexes are intrinsically flexible and often challenging to purify for
structure determination by single-particle cryo-electron microscopy (cryo-EM). Such …

Motile bacteria use chemotaxis to migrate towards environments that are favorable for
growth and survival. The signaling pathway that mediates this behavior is largely conserved …

The proper functioning of biomolecules in living cells requires them to assume particular
structures and to undergo conformational changes. Both biomolecular structure and motion …

Methane-oxidizing bacteria play a central role in greenhouse gas mitigation and have
potential applications in biomanufacturing. Their primary metabolic enzyme, particulate …

Highlights•Cryo-electron tomography (cryoET) subtomogram averaging has emerged as a
structural biology method for sparse and heterogenerous sampls.•CryoET subtomogram …

Highlights•Cryo-ET and subtomogram averaging allow visualizing macromolecular
complexes in close to physiological conditions.•This offers a significant potential for in situ …

Drug molecules adopt a range of conformations both in solution and in their protein-bound
state. The strain and reduced flexibility of bound drugs can partially counter the …

In contrast to Escherichia coli, a model organism for chemotaxis that has 5 chemoreceptors
and a single chemosensory pathway, Pseudomonas aeruginosa PAO1 has a much more …