Expanding the Genetic Code of Bioelectrocatalysis and Biomaterials

Y Chemla, F Kaufman, M Amiram, L Alfonta - Chemical Reviews, 2024 - ACS Publications
Genetic code expansion is a promising genetic engineering technology that incorporates
noncanonical amino acids into proteins alongside the natural set of 20 amino acids. This …

Natural occurring and engineered enzymes for peptide ligation and cyclization

T Nuijens, A Toplak, M Schmidt, A Ricci… - Frontiers in …, 2019 - frontiersin.org
The renaissance of peptides as prospective therapeutics has fostered the development of
novel strategies for their synthesis and modification. In this context, besides the development …

Chemical unfolding of protein domains induces shape change in programmed protein hydrogels

LR Khoury, I Popa - Nature communications, 2019 - nature.com
Programmable behavior combined with tailored stiffness and tunable biomechanical
response are key requirements for developing successful materials. However, these …

High force catch bond mechanism of bacterial adhesion in the human gut

Z Liu, H Liu, AM Vera, RC Bernardi, P Tinnefeld… - Nature …, 2020 - nature.com
Bacterial colonization of the human intestine requires firm adhesion of bacteria to insoluble
substrates under hydrodynamic flow. Here we report the molecular mechanism behind an …

An innovative electrochemical immunosensor based on nanobody heptamer and AuNPs@ ZIF-8 nanocomposites as support for the detection of alpha fetoprotein in …

L Li, X Liu, B Su, H Zhang, R Li, Z Liu, Q Chen… - Microchemical …, 2022 - Elsevier
A novel electrochemical immunoassay based on nanobody heptamer for ultrasensitive
detection of alpha fetoprotein (AFP) is proposed. First, AFP heptamer fusion protein (A1 …

Nanobody multimerization strategy to enhance the sensitivity of competitive ELISA for detection of ochratoxin A in coffee samples

K Bao, X Liu, Q Xu, B Su, Z Liu, H Cao, Q Chen - Food control, 2021 - Elsevier
Enzyme-linked immunosorbent assay (ELISA) is an ideal immunoassay technique for large
scale screening detection of various chemical contaminants in food due to its high …

Broadening the scope of sortagging

X Dai, A Böker, U Glebe - RSC advances, 2019 - pubs.rsc.org
Sortases are enzymes occurring in the cell wall of Gram-positive bacteria. Sortase A (SrtA),
the best studied sortase class, plays a key role in anchoring surface proteins with the …

Building biomaterials through genetic code expansion

V Sisila, M Indhu, J Radhakrishnan, N Ayyadurai - Trends in Biotechnology, 2023 - cell.com
Genetic code expansion (GCE) enables directed incorporation of noncoded amino acids
(NCAAs) and unnatural amino acids (UNAAs) into the active core that confers dedicated …

Elastin‐like recombinamers: deconstructing and recapitulating the functionality of Extracellular Matrix proteins using recombinant protein polymers

S Acosta, L Quintanilla‐Sierra, L Mbundi… - Advanced Functional …, 2020 - Wiley Online Library
In the development of tissue engineering strategies to replace, remodel, regenerate, or
support damaged tissue, the development of bioinspired biomaterials that recapitulate the …

The construction of elastin-like polypeptides and their applications in drug delivery system and tissue repair

Y Guo, S Liu, D Jing, N Liu, X Luo - Journal of Nanobiotechnology, 2023 - Springer
Elastin-like polypeptides (ELPs) are thermally responsive biopolymers derived from natural
elastin. These peptides have a low critical solution temperature phase behavior and can be …