We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and its
recovery in Escherichia coli for the purpose of constructing an in-frame gene deletion in …

This protocol is part of a series of methodologies for the construction of an in-frame gene
deletion in Staphylococcus aureus strain RN4220. Having previously described how an …

Gene deletions can be generated in Staphylococcus aureus using recombineering in
combination with a CRISPR–Cas9 counterselection approach. The method involves first …

Here we describe an allelic-exchange procedure for the construction of an unmarked gene
deletion in the bacterium Staphylococcus aureus. As a practical example, we outline the …

Chromosomal mutations and targeted gene deletions and inactivations in Staphylococcus
aureus are typically generated using the allelic exchange method. In recent years, however …