[HTML][HTML] Combination of whole genome sequencing and metagenomics for microbiological diagnostics
Whole genome sequencing (WGS) provides the highest resolution for genome-based
species identification and can provide insight into the antimicrobial resistance and virulence …
species identification and can provide insight into the antimicrobial resistance and virulence …
Future potential of metagenomics in microbiology laboratories
Rapid and sensitive diagnostic strategies are necessary for patient care and public health.
Most of the current conventional microbiological assays detect only a restricted panel of …
Most of the current conventional microbiological assays detect only a restricted panel of …
Targeted versus shotgun metagenomic sequencing-based detection of microorganisms in sonicate fluid for periprosthetic joint infection diagnosis
Background Next-generation sequencing (NGS) is increasingly used for periprosthetic joint
infection (PJI) diagnosis, but its clinical utility is poorly defined. Shotgun metagenomic …
infection (PJI) diagnosis, but its clinical utility is poorly defined. Shotgun metagenomic …
Pathogen detection in infective endocarditis using targeted metagenomics on whole blood and plasma: a prospective pilot study
Initial microbiologic diagnosis of infective endocarditis (IE) relies on blood cultures and
Bartonella and Coxiella burnetii serology. Small case series and one prospective study have …
Bartonella and Coxiella burnetii serology. Small case series and one prospective study have …
Targeted metagenomic sequencing-based approach applied to 2146 tissue and body fluid samples in routine clinical practice
Background The yield of next-generation sequencing (NGS) added to a Sanger sequencing–
based 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) assay was …
based 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) assay was …
Biological effect abundance analysis of hemolytic pathogens based on engineered biomimetic sensor
Q Yuan, D Mao, X Tang, C Liu, R Zhang, J Deng… - Biosensors and …, 2023 - Elsevier
Conventional pathogen detection strategies based on the molecular structure or chemical
characteristics of biomarkers can only provide the “physical abundance” of microorganisms …
characteristics of biomarkers can only provide the “physical abundance” of microorganisms …
An integrated method for targeted Oxford Nanopore sequencing and automated bioinformatics for the simultaneous detection of bacteria, fungi, and ARG
S Kuruwa, A Zade, S Shah, R Moidu… - Journal of Applied …, 2024 - academic.oup.com
Aims The use of metagenomics for pathogen identification in clinical practice has been
limited. Here we describe a workflow to encourage the clinical utility and potential of NGS for …
limited. Here we describe a workflow to encourage the clinical utility and potential of NGS for …
[HTML][HTML] Identification and characterization of pancreatic infections in severe and critical acute pancreatitis patients using 16S rRNA gene next generation sequencing
N Sun, Y Chen, J Zhang, J Cao, H Huang… - Frontiers in …, 2023 - frontiersin.org
Objectives This study aimed to identify the bacterial composition in the pancreatic fluid of
severe and critical acute pancreatitis (SAP and CAP) patients. Methods A total of 78 …
severe and critical acute pancreatitis (SAP and CAP) patients. Methods A total of 78 …
[HTML][HTML] Diagnosis by microbial culture, breath tests and urinary excretion tests, and treatments of small intestinal bacterial overgrowth
Y Maeda, T Murakami - Antibiotics, 2023 - mdpi.com
Small intestinal bacterial overgrowth (SIBO) is characterized as the increase in the number
and/or alteration in the type of bacteria in the upper gastrointestinal tract and accompanies …
and/or alteration in the type of bacteria in the upper gastrointestinal tract and accompanies …
[HTML][HTML] Clinical application of metagenomic next-generation sequencing in patients with hematologic malignancies suffering from sepsis
WD Liu, TY Yen, PY Liu, UI Wu, P Bhan, YC Li, CH Chi… - Microorganisms, 2021 - mdpi.com
Background: Sepsis remains a common but fatal complication among patients with immune
suppression. We aimed to investigate the performance of metagenomic next-generation …
suppression. We aimed to investigate the performance of metagenomic next-generation …