The biomedical research community addresses reproducibility challenges in animal studies
through standardized nomenclature, improved experimental design, transparent reporting …

Targeted integration of large DNA cargoes (> 10 kb) or genomic replacements in
mammalian cells, such as human pluripotent stem cells (hPS cells), remains challenging …

Glucagon, a hormone released from pancreatic α-cells, is critical for maintaining euglycemia
and plays a key role in the pathophysiology of diabetes. To stimulate the development of …

Perturbation of the excitation/inhibition (E/I) balance leads to neurodevelopmental diseases
including to autism spectrum disorders, intellectual disability, and epilepsy. Loss-of-function …

Adequate species' identification is critical for the detection and monitoring of biological
invasions. In this study, we proposed and assessed the efficiency of newly created primer …

Sialic acid-binding immunoglobulin-like lectins (Siglecs) are important immunomodulatory
receptors. Due to differences between human and mouse Siglecs, defining the in vivo roles …

Simple Summary We developed a method to detect a single-point mutation in an obese
gene in a mouse research model. The method is quick, easy to conduct, and suitable for …

Gene targeting and additive (random) transgenesis have proven to be powerful
technologies with which to decipher the mammalian genome. With the advent of …

Background Host-related factors highly regulate the increased circulation of neutrophils
during Leishmania infection. Platelet-derived Dickkopf-1 (DKK1) is established as a high …

Twitcher (Twi) is a neurological Krabbe disease (KD, or globoid cell leukodystrophy)
spontaneous mutant line in mice. The genome of the Twi mouse presents a single …