Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of
complex molecules with diverse applications, from medicine to agriculture and materials …

Cells navigate environments, communicate and build complex patterns by initiating gene
expression in response to specific signals. Engineers seek to harness this capability to …

Base editors use DNA-modifying enzymes targeted with a catalytically impaired CRISPR
protein to precisely install point mutations. Here, we develop phage-assisted continuous …

The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of
measurements of rapid processes such as gene expression dynamics and effectively …

Among the most urgent public health threats is the worldwide emergence of carbapenem-
resistant Enterobacteriaceae,,–, which are resistant to the antibiotic class of 'last resort'. In …

Isoprenoids comprise a large class of chemicals of significant interest due to their diverse
properties. Biological production of isoprenoids is considered to be the most efficient way for …

An inability to reliably predict quantitative behaviors for novel combinations of genetic
elements limits the rational engineering of biological systems. We developed an expression …

Cells can be programmed to monitor and react to their environment using genetic circuits.
Design automation software maps a desired circuit function to a DNA sequence, a process …

Logic and memory are essential functions of circuits that generate complex, state-dependent
responses. Here we describe a strategy for efficiently assembling synthetic genetic circuits …

The past several years have witnessed an increased presence of control theoretic concepts
in synthetic biology. This review presents an organized summary of how these control …