Thermozymes: adaptive strategies and tools for their biotechnological applications
In today's scenario of global climate change, there is a colossal demand for sustainable
industrial processes and enzymes from thermophiles. Plausibly, thermozymes are an …
industrial processes and enzymes from thermophiles. Plausibly, thermozymes are an …
Orthogonal Translation for Site-Specific Installation of Post-translational Modifications
Q Gan, C Fan - Chemical Reviews, 2024 - ACS Publications
Post-translational modifications (PTMs) endow proteins with new properties to respond to
environmental changes or growth needs. With the development of advanced proteomics …
environmental changes or growth needs. With the development of advanced proteomics …
Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase
Pyrrolysyl-tRNA synthetase (PylRS) is a major tool in genetic code expansion using
noncanonical amino acids, yet its structure and function are not completely understood …
noncanonical amino acids, yet its structure and function are not completely understood …
Ancestral archaea expanded the genetic code with pyrrolysine
The pyrrolysyl-tRNA synthetase (PylRS) facilitates the cotranslational installation of the 22nd
amino acid pyrrolysine. Owing to its tolerance for diverse amino acid substrates, and its …
amino acid pyrrolysine. Owing to its tolerance for diverse amino acid substrates, and its …
Expanding the Genetic Code of Xenopus laevis Embryos
The incorporation of unnatural amino acids into proteins through genetic code expansion
has been successfully adapted to African claw-toed frog embryos. Six unique unnatural …
has been successfully adapted to African claw-toed frog embryos. Six unique unnatural …
Directed Evolution of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Generates a Hyperactive and Highly Selective Variant
Pyrrolysyl-tRNA synthetase (PylRS) is frequently used for site-specific incorporation of
noncanonical amino acids (ncAAs) into proteins. Recently, the active site of …
noncanonical amino acids (ncAAs) into proteins. Recently, the active site of …
Recent development of genetic code expansion for posttranslational modification studies
Nowadays advanced mass spectrometry techniques make the identification of protein
posttranslational modifications (PTMs) much easier than ever before. A series of proteomic …
posttranslational modifications (PTMs) much easier than ever before. A series of proteomic …
Structural basis for genetic-code expansion with bulky lysine derivatives by an engineered pyrrolysyl-tRNA synthetase
T Yanagisawa, M Kuratani, E Seki, N Hino… - Cell Chemical …, 2019 - cell.com
Pyrrolysyl-tRNA synthetase (PylRS) and tRNA Pyl have been extensively used for genetic-
code expansion. A Methanosarcina mazei PylRS mutant bearing the Y306A and Y384F …
code expansion. A Methanosarcina mazei PylRS mutant bearing the Y306A and Y384F …
Engineering of enzymes using non-natural amino acids
In enzyme engineering, the main targets for enhancing properties are enzyme activity,
stereoselective specificity, stability, substrate range, and the development of unique …
stereoselective specificity, stability, substrate range, and the development of unique …
Understanding the genetic code
MH Saier Jr - Journal of bacteriology, 2019 - Am Soc Microbiol
The universal triple-nucleotide genetic code is often viewed as a given, randomly selected
through evolution. However, as summarized in this article, many observations and …
through evolution. However, as summarized in this article, many observations and …