Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer bacterium to become a flexible and engineer-able platform for metabolic engineering stems …
Abstract The SEVA platform (https://seva-plasmids. com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …
Polyhydroxyalkanoates (PHA) are microbial polyesters produced by numerous prokaryotes. These materials are generally considered to be renewable and biodegradable alternatives …
Legumes obtain nitrogen from air through rhizobia residing in root nodules. Some species of rhizobia can colonize cereals but do not fix nitrogen on them. Disabling native regulation …
Abstract The Standard European Vector Architecture 3.0 database (SEVA-DB 3.0, http://seva. cnb. csic. es) is the update of the platform launched in 2013 both as a web-based …
P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically …