Grand challenges for industrializing polyhydroxyalkanoates (PHAs)

D Tan, Y Wang, Y Tong, GQ Chen - Trends in biotechnology, 2021 - cell.com
Polyhydroxyalkanoates (PHAs) are a diverse family of sustainable bioplastics synthesized
by various bacteria, but their high production cost and unstable material properties make …

Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria

DC Volke, E Orsi, PI Nikel - Current Opinion in Microbiology, 2023 - Elsevier
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes

E Martínez-García, S Fraile, E Algar… - Nucleic Acids …, 2023 - academic.oup.com
Abstract The SEVA platform (https://seva-plasmids. com) was launched one decade ago,
both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …

Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

DC Volke, RA Martino, E Kozaeva, AM Smania… - Nature …, 2022 - nature.com
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics

S Obruča, P Dvořák, P Sedláček, M Koller… - Biotechnology …, 2022 - Elsevier
Polyhydroxyalkanoates (PHA) are microbial polyesters produced by numerous prokaryotes.
These materials are generally considered to be renewable and biodegradable alternatives …

Control of nitrogen fixation in bacteria that associate with cereals

MH Ryu, J Zhang, T Toth, D Khokhani, BA Geddes… - Nature …, 2020 - nature.com
Legumes obtain nitrogen from air through rhizobia residing in root nodules. Some species of
rhizobia can colonize cereals but do not fix nitrogen on them. Disabling native regulation …

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts

E Martínez-García, A Goni-Moreno… - Nucleic acids …, 2020 - academic.oup.com
Abstract The Standard European Vector Architecture 3.0 database (SEVA-DB 3.0,
http://seva. cnb. csic. es) is the update of the platform launched in 2013 both as a web-based …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …