Protein fusion tags are indispensible tools used to improve recombinant protein expression
yields, enable protein purification, and accelerate the characterization of protein structure …

Publisher Summary This chapter discusses DNA transformation. DNA transformation assays
in a whole organism provide experimental links between molecular structure and …

Specialized epitope tags are widely used for detecting, manipulating or purifying proteins,
but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed …

Genetic-based methods offer environmentally friendly species-specific approaches for
control of insect pests. One method, CRISPR homing gene drive that target genes essential …

An important recent advance in the functional analysis of Saccharomyces cerevisiae genes
is the development of the one‐step PCR‐mediated technique for deletion and modification …

Cerebral deposition of amyloid β peptide (Aβ) is an early and critical feature of Alzheimer's
disease. Aβ generation depends on proteolytic cleavage of the amyloid precursor protein …

We describe a straightforward PCR‐based approach to the deletion, tagging, and
overexpression of genes in their normal chromosomal locations in the fission yeast …

Rare individuals have been multiply exposed to HIV-1 but remain uninfected. The CD4+ T-
cells of two of these individuals, designated EU2 and EU3, are highly resistant in vitro to the …

Human papillomavirus type 16 (HPV-16) is a DNA tumor virus that is associated with human
anogenital cancers and encodes two transforming proteins, E6 and E7. The E7 protein has …

2012-05-08 Assigned to RAINDANCE TECHNOLOGIES, INC. reassignment RAINDANCE
TECHNOLOGIES, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …