Highlights•Guidelines for in-house and inter-laboratory validation of qualitative qPCR
methods.•Method acceptance and performance parameters to be evaluated.•Acceptance …

In many countries, genetically modified organisms (GMO) legislations have been
established in order to guarantee the traceability of food/feed products on the market and to …

Biotech crops are the fastest adopted crop technology in the history of modern agriculture.
The commercialisation of GMO is in many countries strictly regulated laying down the need …

Recently, we developed a DNA walking system for the detection and characterization of a
broad spectrum of GMOs in routine analysis of food/feed matrices. Here, we present a new …

The current study was conducted to statistically compare the SYBR® Green quantitative
polymerase chain reaction (qPCR) assay and the conventional plate counting (PC) method …

In the coming years, the frequency of unauthorised genetically modified organisms (GMOs)
being present in the European food and feed chain will increase significantly. Therefore, we …

To identify unauthorised GMOs in food and feed matrices, an integrated approach has
recently been developed targeting pCAMBIA family vectors, highly present in transgenic …

In order to characterize unauthorized genetically modified petunia, an integrated strategy
has been applied here on several suspected petunia samples from the European market …

Background In order to provide a system fully integrated with qPCR screening, usually used
in GMO routine analysis, as well as being able to detect, characterize and identify a broad …

Abstract Combinatory SYBR® Green real-time PCR Screening (CoSYPS) is an efficient,
sensitive approach for detecting complex targets such as genetically modified organisms …