CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns

CNT Taning, B Van Eynde, N Yu, S Ma… - Journal of insect …, 2017 - Elsevier
Discovered as a bacterial adaptive immune system, CRISPR/Cas9 (clustered, regularly
interspaced, short palindromic repeat/CRISPR associated) is being developed as an …

CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

JJ Li, Y Shi, JN Wu, H Li, G Smagghe, TX Liu - Journal of insect physiology, 2021 - Elsevier
Clustered regularly spaced short palindrome repeats (CRISPR) structure family forms the
acquired immune system in bacteria and archaea. Recent advances in CRISPR/Cas …

A fungal pathogen deploys a small silencing RNA that attenuates mosquito immunity and facilitates infection

C Cui, Y Wang, J Liu, J Zhao, P Sun, S Wang - Nature communications, 2019 - nature.com
Insecticidal fungi represent a promising alternative to chemical pesticides for disease vector
control. Here, we show that the pathogenic fungus Beauveria bassiana exports a microRNA …

The miR-9b microRNA mediates dimorphism and development of wing in aphids

F Shang, J Niu, BY Ding, W Zhang… - Proceedings of the …, 2020 - National Acad Sciences
Wing dimorphism is a phenomenon of phenotypic plasticity in aphid dispersal. However, the
signal transduction for perceiving environmental cues (eg, crowding) and the regulation …

Post-transcriptional regulation of insect metamorphosis and oogenesis

J Song, S Zhou - Cellular and Molecular Life Sciences, 2020 - Springer
Metamorphic transformation from larvae to adults along with the high fecundity is key to
insect success. Insect metamorphosis and reproduction are governed by two critical …

The RNase III enzyme Dicer1 is essential for larval development in Bombyx mori

D Chen, X Yang, D Yang, Y Liu, Y Wang, X Luo… - Insect …, 2023 - Wiley Online Library
MicroRNAs (miRNAs) are important regulators of nearly all aspects of biological processes
in eukaryotes. During the biogenesis of miRNAs, the RNase III enzyme Dicer processes …

Clustered miR-2, miR-13a, miR-13b and miR-71 coordinately target Notch gene to regulate oogenesis of the migratory locust Locusta migratoria

J Song, W Li, H Zhao, S Zhou - Insect biochemistry and molecular biology, 2019 - Elsevier
Abstract MicroRNAs (miRNAs),∼ 22-nt small noncoding RNAs with a crucial role in various
biological processes of organisms, are usually clustered in the genome. However, little is …

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

GM Baci, AA Cucu, AI Giurgiu, AS Muscă, L Bagameri… - Insects, 2021 - mdpi.com
Simple Summary One of the most powerful gene editing approaches is the CRISPR
(clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) tool …

[PDF][PDF] A practical guide to CRISPR/Cas9 genome editing in Lepidoptera

L Zhang, RD Reed - Diversity and evolution of butterfly wing …, 2017 - library.oapen.org
CRISPR/Cas9 genome editing has revolutionized functional genetic work in many
organisms and is having an especially strong impact in emerging model systems. Here we …

MicroRNA-14 regulates larval development time in Bombyx mori

Z Liu, L Ling, J Xu, B Zeng, Y Huang, P Shang… - Insect Biochemistry and …, 2018 - Elsevier
MicroRNAs (miRNA) regulate multiple physiological processes including development and
metamorphosis in insects. In the current study, we demonstrate that a conserved …