Next-generation sequence assembly: four stages of data processing and computational challenges

S El-Metwally, T Hamza, M Zakaria… - PLoS computational …, 2013 - journals.plos.org
Decoding DNA symbols using next-generation sequencers was a major breakthrough in
genomic research. Despite the many advantages of next-generation sequencers, eg, the …

An evaluation of the PacBio RS platform for sequencing and de novo assembly of a chloroplast genome

M Ferrarini, M Moretto, JA Ward, N Šurbanovski… - BMC genomics, 2013 - Springer
Background Second generation sequencing has permitted detailed sequence
characterisation at the whole genome level of a growing number of non-model organisms …

Phage annotation guide: guidelines for assembly and high-quality annotation

D Turner, EM Adriaenssens, I Tolstoy, AM Kropinski - Phage, 2021 - liebertpub.com
All sequencing projects of bacteriophages (phages) should seek to report an accurate and
comprehensive annotation of their genomes. This article defines 14 questions for those new …

Computational approaches for transcriptome assembly based on sequencing technologies

Y Luo, X Liao, FX Wu, J Wang - Current Bioinformatics, 2020 - benthamdirect.com
Transcriptome assembly plays a critical role in studying biological properties and examining
the expression levels of genomes in specific cells. It is also the basis of many downstream …

In vitro and in silico based approaches to identify potential novel bacteriocins from the athlete gut microbiome of an elite athlete cohort

L Wosinska, CJ Walsh, PM O'Connor, EM Lawton… - Microorganisms, 2022 - mdpi.com
Exercise reduces inflammation, fatigue, and aids overall health. Additionally, physical fitness
has been associated with desirable changes in the community composition of the athlete gut …

Identification of key genes and molecular mechanisms associated with temperature stress in lentil

SS Sohrabi, A Ismaili, F Nazarian-Firouzabadi… - Gene, 2022 - Elsevier
Extreme temperature is one of the serious threats to crop production in present and future
scenarios of global climate changes. Lentil (Lens culinaris) is an important crop, and there is …

Enhanced whole genome sequence and annotation of Clostridium stercorarium DSM8532T using RNA-seq transcriptomics and high-throughput proteomics

JJ Schellenberg, TJ Verbeke, P McQueen, OV Krokhin… - BMC genomics, 2014 - Springer
Background Growing interest in cellulolytic clostridia with potential for consolidated biofuels
production is mitigated by low conversion of raw substrates to desired end products …

[HTML][HTML] Beyond the whole genome consensus: unravelling of PRRSV phylogenomics using next generation sequencing technologies

ZH Lu, AL Archibald, T Ait-Ali - Virus research, 2014 - Elsevier
The highly heterogeneous porcine reproductive and respiratory syndrome virus (PRRSV) is
the causative agent responsible for an economically important pig disease with the …

Refinement of Draft Genome Assemblies of Pigeonpea (Cajanus cajan)

SS Marla, P Mishra, R Maurya, M Singh… - Frontiers in …, 2020 - frontiersin.org
Genome assembly of short reads from large plant genomes remains a challenge in
computational biology despite major developments in next generation sequencing. Of late …

Prospects of Bioinformatics and Data Acquirement Tools in Boosting the Application of Phytochemicals in Food Sciences

AK Jaiswal, PP Singh, B Prakash - Plant Essential Oils: From Traditional to …, 2023 - Springer
Bioinformatics has been established as a multidisciplinary research area bringing about a
paradigm shift in the discipline of biological researches. In silico resources made biologists …