In recent years, powerful genetic code reprogramming methods have emerged that allow
new functional components to be embedded into proteins as noncanonical amino acid …

Our understanding of the complex molecular processes of living organisms at the molecular
level is growing exponentially. This knowledge, together with a powerful arsenal of tools for …

Interrogating biological systems is often limited by access to biological probes. The
emergence of “click chemistry” has revolutionized bioconjugate chemistry by providing facile …

The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

The goal of xenobiology is to design biological systems endowed with unusual biochemical
functions, whereas enzymology concerns the study of enzymes, the workhorses of …

Breakthroughs in enzyme immobilization have enabled increased enzyme recovery and
reusability, leading to significant decreases in the cost of enzyme use and fueling …

Biomaterials based on immobilized proteins are key elements of many biomedical and
industrial technologies. However, applications are limited by an inability to precisely …

In enzyme engineering, the main targets for enhancing properties are enzyme activity,
stereoselective specificity, stability, substrate range, and the development of unique …

Herein we describe a simple protocol for the efficient generation of site-specific ubiquitin-
protein conjugates using click chemistry. By using two different methods to expand the …

Albumin fusion/conjugation (albumination) has been an effective method to prolong in vivo
half-life of therapeutic proteins. However, its broader application to proteins with complex …