Transfer RNA is an essential adapter molecule that is found across all three domains of life.
The primary role of transfer RNA resides in its critical involvement in the accurate translation …

Aminoacylation of tRNAs, catalyzed by 20 aminoacyl-tRNA synthetases, is responsible for
establishing the genetic code. The enzymes are divided into two classes on the basis of the …

Dynamic light scattering (DLS) analyses are routinely used in biology laboratories to detect
aggregates in macromolecular solutions, to determine the size of proteins, nucleic acids …

Abstract Aminoacyl-tRNAs (aa-tRNAs) are the essential substrates for translation. Most aa-
tRNAs are formed by direct aminoacylation of tRNA catalyzed by aminoacyl-tRNA …

The amide proteogenic amino acids, asparagine and glutamine, are two of the twenty amino
acids used in translation by all known life. The aminoacyl‐tRNA synthetases for asparagine …

Aminoacyl-tRNA synthetases (aaRSs) are modular enzymes globally conserved in the three
kingdoms of life. All catalyze the same two-step reaction, ie, the attachment of a …

Biochemical experiments and genomic sequence analysis showed that Deinococcus
radiodurans and Thermus thermophilus do not possess asparagine synthetase (encoded by …

Aminoacyl-tRNA is generally formed by aminoacyl-tRNA synthetases, a family of 20
enzymes essential for accurate protein synthesis. However, most bacteria generate one of …

Evolutionary inferences rely on diversity. The source of differences among organisms is
accumulated divergence from a common ancestor, which may be random or selected. When …

Thermus filiformis is an aerobic thermophilic bacterium isolated from a hot spring in New
Zealand. The experimental study of the mechanisms of thermal adaptation is important to …