Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …

Dynamic alterations in the unique brain extracellular matrix (ECM) are involved in malignant
brain tumors. Yet studies of brain ECM roles in tumor cell behavior have been difficult due to …

Background Glioblastoma (GB) is the most severe form of brain cancer, with a 12-15 month
median survival. Surgical resection, temozolomide (TMZ) treatment, and radiotherapy …

Epithelial ovarian cancer is the deadliest gynecologic cancer, due in large part to recurrent
tumors. Recurrences tend to have metastasized, mainly in the peritoneal cavity and …

The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral
fluorescence imaging, has increased the use of these techniques, and improved the ease …

The availability of energy has significant impact on cell physiology. However, the role of
cellular metabolism in bacterial pathogenesis is not understood. We investigated the …

The excited-state lifetime is an intrinsic property of fluorescent molecules that can be
leveraged for multiplexed imaging. An advantage of fluorescence lifetime-based …

Background The existing cell surface markers used for sorting glioma stem cells (GSCs)
have obvious limitations, such as vulnerability to the enzymatic digestion and time …

Maximal safe tumor resection remains the key prognostic factor for improved prognosis in
brain tumor patients. Despite 5-aminolevulinic acid-based fluorescence guidance the …