In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal
activity in the intact brain. It is based on the principle that, when neurons fire action …

Tremendous progress has been made since Neuron published our Primer on genetic
dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical …

Mapping behavioural actions to neural activity is a fundamental goal of neuroscience. As our
ability to record large neural and behavioural data increases, there is growing interest in …

Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics
with cell-type specificity. However, current indicators enable a narrow range of applications …

To test the hypothesized crucial role of microglia in the developmental refinement of neural
circuitry, we depleted microglia from mice of both sexes with PLX5622 and examined the …

Calcium imaging has transformed neuroscience research by providing a methodology for
monitoring the activity of neural circuits with single-cell resolution. However, calcium …

Calcium imaging with fluorescent protein sensors is widely used to record activity in
neuronal populations. The transform between neural activity and calcium-related …

The past decade has witnessed remarkable advances in the simultaneous measurement of
neuronal activity across many brain regions, enabling fundamentally new explorations of the …

Fluorescent calcium indicators are often used to investigate neural dynamics, but the
relationship between fluorescence and action potentials (APs) remains unclear. Most APs …

Inference of action potentials ('spikes') from neuronal calcium signals is complicated by the
scarcity of simultaneous measurements of action potentials and calcium signals ('ground …