Functional solubilization of the β2-adrenoceptor using diisobutylene maleic acid
Summary The β2-adrenoceptor (β2AR) is a well-established target in asthma and a
prototypical G protein-coupled receptor for biophysical studies. Solubilization of membrane …
prototypical G protein-coupled receptor for biophysical studies. Solubilization of membrane …
ThermoBRET: a ligand-engagement nanoscale thermostability assay applied to GPCRs
Measurements of membrane protein thermostability allows indirect detection of ligand
binding. Current thermostability assays require protein purification or rely on pre-existing …
binding. Current thermostability assays require protein purification or rely on pre-existing …
[PDF][PDF] Measurement of non-purified GPCR thermostability using the homogenous ThermoBRET assay
BL Hoare, A Kaur, CR Harwood, NC Dijon, ND Holliday… - bioRxiv, 2020 - researchgate.net
Sensitive assays to measure the thermostability of membrane proteins are important tools in
protein purification optimisation and drug discovery. Here, we present a ThermoBRET …
protein purification optimisation and drug discovery. Here, we present a ThermoBRET …