The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …

Efficient and reproducible transgenesis facilitates and accelerates research using genetic
model organisms. Here, we describe a mo dular s afe-harbor t ransgene i nsertion (MosTI) …

CRISPR-based genome-editing methods in model organisms are evolving at an
extraordinary speed. Whereas the generation of deletion or missense mutants is quite …

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE)
system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis …

GFP labeling by genome editing can reveal the authentic location of a native protein, but is
frequently hampered by weak GFP signals and broad expression across a range of tissues …

The sense of touch is conferred by the conjoint function of somatosensory neurons and skin
cells. These cells meet across a gap filled by a basal lamina, an ancient structure found in …

Precision genome editing for model organisms has revolutionized functional analysis and
validation of a wide variety of molecular systems. To date, the capacity to insert single-copy …

Members of the superfamily of solute carrier (SLC) transmembrane proteins transport
diverse substrates across distinct cellular membranes. Three SLC protein families transport …

Successful transgenesis in model organisms has dramatically helped us understand gene
function, regulation, genetic networks, and potential applications. Here, we introduce the …