The packaging of DNA into strings of nucleosomes is one of the features that allows
eukaryotic cells to tightly regulate gene expression. The ordered disassembly of …

In eukaryotic cells, protein and RNA factors involved in genome activities like transcription,
RNA processing, DNA replication, and repair accumulate in self-organizing membraneless …

During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

Chromatin landscapes are disrupted during DNA replication and must be restored faithfully
to maintain genome regulation and cell identity. The histone H3-H4 modification landscape …

Cells alter their mechanical properties in response to their local microenvironment; this plays
a role in determining cell function and can even influence stem cell fate. Here, we identify a …

The eukaryotic genome is organized within cells as chromatin. For proper information
output, higher-order chromatin structures can be regulated dynamically. How such structures …

During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the
help of various elongation factors. Here, we show that RNA Pol II achieves efficient …

Although SIRT7 is a member of sirtuin family proteins that are described as NAD+-
dependent class III histone deacetylases, the intrinsic enzymatic activity of this sirtuin protein …

Transcription in several organisms from certain bacteria to humans has been observed to be
stochastic in nature: toggling between active and inactive states. Periods of active nascent …

Among the diverse transcription factors that are necessary to elicit changes in cell fate, both
in embryonic development and in cellular reprogramming, a subset of factors are capable of …