The SARS-CoV-2 nucleocapsid protein and its role in viral structure, biological functions, and a potential target for drug or vaccine mitigation

Z Bai, Y Cao, W Liu, J Li - Viruses, 2021 - mdpi.com
The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the world
is still expanding. Thus, there is an urgent need to better understand this novel virus and find …

Liquid–liquid phase separation in disease

S Alberti, D Dormann - Annual review of genetics, 2019 - annualreviews.org
We have made rapid progress in recent years in identifying the genetic causes of many
human diseases. However, despite this recent progress, our mechanistic understanding of …

Genomic RNA elements drive phase separation of the SARS-CoV-2 nucleocapsid

C Iserman, CA Roden, MA Boerneke, RSG Sealfon… - Molecular cell, 2020 - cell.com
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid
phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic …

SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

TM Perdikari, AC Murthy, VH Ryan, S Watters… - The EMBO …, 2020 - embopress.org
Tightly packed complexes of nucleocapsid protein and genomic RNA form the core of
viruses and assemble within viral factories, dynamic compartments formed within the host …

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

A Savastano, A Ibáñez de Opakua, M Rankovic… - Nature …, 2020 - nature.com
The etiologic agent of the Covid-19 pandemic is the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2). The viral membrane of SARS-CoV-2 surrounds a helical …

Lipid droplets fuel SARS-CoV-2 replication and production of inflammatory mediators

SSG Dias, VC Soares, AC Ferreira… - PLoS …, 2020 - journals.plos.org
Viruses are obligate intracellular parasites that make use of the host metabolic machineries
to meet their biosynthetic needs. Thus, identifying the host pathways essential for the virus …

Negri bodies are viral factories with properties of liquid organelles

J Nikolic, R Le Bars, Z Lama, N Scrima… - Nature …, 2017 - nature.com
Replication of Mononegavirales occurs in viral factories which form inclusions in the host-
cell cytoplasm. For rabies virus, those inclusions are called Negri bodies (NBs). We report …

Implications of oxidative stress and potential role of mitochondrial dysfunction in COVID-19: therapeutic effects of vitamin D

N de Las Heras, VM Martín Giménez, L Ferder… - Antioxidants, 2020 - mdpi.com
Due to its high degree of contagiousness and like almost no other virus, SARS-CoV-2 has
put the health of the world population on alert. COVID-19 can provoke an acute inflammatory …

Vimentin as a multifaceted player and potential therapeutic target in viral infections

I Ramos, K Stamatakis, CL Oeste… - International journal of …, 2020 - mdpi.com
Vimentin is an intermediate filament protein that plays key roles in integration of cytoskeletal
functions, and therefore in basic cellular processes such as cell division and migration …

Composition and three-dimensional architecture of the dengue virus replication and assembly sites

S Welsch, S Miller, I Romero-Brey, A Merz, CKE Bleck… - Cell host & …, 2009 - cell.com
Positive-strand RNA viruses are known to rearrange cellular membranes to facilitate viral
genome replication. The biogenesis and three-dimensional organization of these …