Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

Superresolution fluorescence microscopy permits the study of biological processes at scales
small enough to visualize fine subcellular structures that are unresolvable by traditional …

In this Perspective we discuss recent trends in the development and applications of
fluorescent proteins. We start by providing a historical and structural perspective of their …

Highlights•Photoswitchable fluorescent proteins cycle between on and off states in response
to light.•Structural studies reveal diversity in the details of photoswitching …

Development of single-molecule localization microscopy (SMLM) has sparked a revolution
in biological imaging, allowing “super-resolution” fluorescence microscopy below the …

Super-resolution microscopy is the term commonly given to fluorescence microscopy
techniques with resolutions that are not limited by the diffraction of light. Since their …

Phototransformable fluorescent proteins (FPs) have received considerable attention in
recent years, because they enable many new exciting modalities in fluorescence …

Optical fluorescence microscopy plays a pivotal role in the exploration of biological structure
and dynamics, especially on live specimens. Progress in the field relies, on the one hand, on …

“Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for
advanced fluorescence imaging. However, only a limited number of such labels is available …

Super-resolution fluorescence microscopy has become an invaluable, powerful approach to
study biomolecular dynamics and interactions via selective labeling and observation of …