Errors occurring during DNA replication can result in inaccurate replication, incomplete
replication, or re-replication, resulting in genome instability that can lead to diseases such as …

The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol)
α–primase initiates each synthesis event and is rapidly replaced by processive DNA …

Single-strand breaks (SSBs) are one of the most commonly occurring endogenous lesions
with the potential to give rise to cytotoxic double-strand breaks (DSBs) during DNA …

Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the
replication dynamics of individual molecules with existing methods. We report a sequencing …

Genomic DNA replicates in a choreographed temporal order that impacts the distribution of
mutations along the genome. We show here that DNA replication timing is shaped by …

Bacteria with multiple chromosomes represent up to 10% of all bacterial species. Unlike
eukaryotes, these bacteria use chromosome-specific initiators for their replication. In all …

Fundamental aspects of DNA replication, such as the anatomy of replication stall sites, how
replisomes are influenced by gene transcription, and whether the progression of sister …

Current temporal studies of DNA replication are either low-resolution or require complex cell
synchronisation and/or sorting procedures. Here we introduce Nanotiming, a single …

Mammalian DNA replication is regulated via multi-replicon segments that replicate in a
defined temporal order during S-phase. Further, early/late replication of RDs corresponds to …