Real-time PCR applications for diagnosis of leishmaniasis

L Galluzzi, M Ceccarelli, A Diotallevi, M Menotta… - Parasites & vectors, 2018 - Springer
Leishmaniasis is a vector-borne disease caused by many Leishmania species, which can
infect both humans and other mammals. Leishmaniasis is a complex disease, with …

Molecular diagnosis of visceral leishmaniasis

S Sundar, OP Singh - Molecular diagnosis & therapy, 2018 - Springer
Visceral leishmaniasis (VL), a deadly parasitic disease, is a major public health concern
globally. Countries affected by VL have signed the London Declaration on Neglected …

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

I Gow, NC Smith, D Stark, J Ellis - Parasites & Vectors, 2022 - Springer
Leishmania infections span a range of clinical syndromes and impact humans from many
geographic foci, but primarily the world's poorest regions. Transmitted by the bite of a female …

Variable gene expression and parasite load predict treatment outcome in cutaneous leishmaniasis

CF Amorim, FO Novais, BT Nguyen, AM Misic… - Science translational …, 2019 - science.org
Patients infected with Leishmania braziliensis develop chronic lesions that often fail to
respond to treatment with antiparasite drugs. To determine whether genes whose …

Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein

LH Patiño, N Ballesteros, M Muñoz, J Jaimes… - Parasites & …, 2023 - Springer
Background Leishmaniasis is a parasitic disease caused by obligate intracellular protozoa
of the genus Leishmania. This infection is characterized by a wide range of clinical …

Novel CRISPR-based detection of Leishmania species

E Dueñas, JA Nakamoto, L Cabrera-Sosa… - Frontiers in …, 2022 - frontiersin.org
Tegumentary leishmaniasis, a disease caused by protozoan parasites of the genus
Leishmania, is a major public health problem in many regions of Latin America. Its diagnosis …

Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis

CPB Filgueira, OC Moreira… - PLoS neglected …, 2020 - journals.plos.org
Leishmaniasis is a worldwide neglected disease, encompassing asymptomatic infections
and different clinical forms, such as American Tegumentary Leishmaniasis (ATL) which is …

Epidemiological features of Leishmania infantum in dogs (Canis lupus familiaris) suggest a latent risk of visceral leishmaniasis in the metropolitan area of …

J Jaimes-Dueñez, A Castillo-Castañeda… - Preventive Veterinary …, 2023 - Elsevier
Visceral leishmaniasis (VL) is a disease caused by species of the Leishmania donovani
complex that is mainly transmitted through the urban cycle involving dogs as the primary …

[HTML][HTML] Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis

JD Ramírez, L Cao, AC Castillo-Castañeda… - Practical Laboratory …, 2023 - Elsevier
Leishmaniasis is a complex vector-borne disease caused by various Leishmania species,
affecting humans and animals. Current diagnostic methods have limitations, leading to …

Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

C Chicharro, J Nieto, S Miguelañez, E Garcia… - Microbiology …, 2023 - Am Soc Microbiol
Leishmaniasis is an endemic parasitic disease in at least 98 countries. In Spain, it is
considered a zoonosis caused by Leishmania infantum, with an annual incidence of 0.62 …