The collagen network is altered in fibrotic diseases associated with extracellular matrix
(ECM) biosynthesis and remodeling. This mini-review focuses on the quantitative and …

Second‐harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and
probing its hierarchical organization from molecular scale up to tissue architectural level. In …

Raman spectroscopy in combination with chemometrics is gaining more and more
importance for answering biological questions. This results from the fact that Raman …

Although chemists, biochemists, biologists, and material scientists are often interested in
using nonlinear optical techniques for characterizing their samples, they seldom have the …

In this tutorial I analyze the polarization-dependent properties of different optical contrasts
widely used today in imaging, applied to biology and biomedical diagnostics. I derive the …

OBJECTIVE Glioblastoma (GBM) is the most malignant primary brain tumor. Collagen is
present in low amounts in normal brain, but in GBMs, collagen gene expression is reportedly …

Type II collagen fibril diameters in cartilage are beneath the diffraction limit of optical
microscopy, which makes the assessment of collagen organization very challenging. In this …

Quantitative second-harmonic generation imaging is employed to assess stromal collagen
in normal, hyperplastic, dysplastic, and malignant breast tissues. The cellular scale …

Collagen (type I) fibers are readily visualized with second harmonic generation (SHG)
microscopy though the molecular origin of the signal has not yet been elucidated. In this …

Second-harmonic generation (SHG) microscopy began to emerge as a high-resolution
optical imaging modality in the late 1990s, about 10 years after two-photon excited …