Past, present and future applications of flow cytometry in aquatic microbiology

Y Wang, F Hammes, K De Roy, W Verstraete… - Trends in …, 2010 - cell.com
Flow cytometry (FCM) is rapidly becoming an essential tool in the field of aquatic
microbiology. It provides opportunities for microbial analysis at both the community and …

Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH

EY Fernando, SJ McIlroy, M Nierychlo… - The ISME …, 2019 - academic.oup.com
Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological
process and relies on the massive accumulation of phosphate within special …

Applications and challenges for single-bacteria analysis by flow cytometry

L Wu, S Wang, Y Song, X Wang, X Yan - Science China Chemistry, 2016 - Springer
Flow cytometry (FCM) is a powerful technique for single-bacteria analysis via simultaneous
light-scattering and fluorescence measurements. By offering high-throughput, quantitative …

Polyhydroxybutyrate and glycogen production in photobioreactors inoculated with wastewater borne cyanobacteria monocultures

E Rueda, MJ García-Galán, R Díez-Montero, J Vila… - Bioresource …, 2020 - Elsevier
The aim of this study was to investigate the PHB and glycogen accumulation dynamics in
two photobioreactors inoculated with different monocultures of wastewater-borne …

Flow cytometric assessment of bacterial abundance in soils, sediments and sludge

A Frossard, F Hammes, MO Gessner - Frontiers in microbiology, 2016 - frontiersin.org
Bacterial abundance is a fundamental measure in microbiology, but its assessment is often
tedious, especially for soil, and sediment samples. To overcome this limitation, we adopted a …

Rapid inactivation of airborne bacteria using atmospheric pressure dielectric barrier grating discharge

MJ Gallagher, N Vaze, S Gangoli… - … on Plasma Science, 2007 - ieeexplore.ieee.org
Dielectric barrier discharge plasma has been known to inactivate many different
microorganisms on surfaces when treatment times are on the order of seconds or minutes in …

Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene …

O Zemb, CS Achard, J Hamelin… - …, 2020 - Wiley Online Library
Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial
communities, by estimating the relative abundance of microbes. Here, we present a method …

Direct quantification of bacterial biomass in influent, effluent and activated sludge of wastewater treatment plants by using flow cytometry

P Foladori, L Bruni, S Tamburini, G Ziglio - Water research, 2010 - Elsevier
A rapid multi-step procedure, potentially amenable to automation, was proposed for
quantifying viable and active bacterial cells, estimating their biovolume using flow cytometry …

Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?

E Velastegui, J Quezada, K Guerrero… - Applied Microbiology …, 2023 - Springer
Culture medium heterogeneity is inherent in industrial bioreactors. The loss of mixing
efficiency in a large-scale bioreactor yields to the formation of concentration gradients …

Validation of 16S rRNA gene sequencing and metagenomics for evaluating microbial immigration in a methanogenic bioreactor

TY Lin, WT Liu - Water Research, 2023 - Elsevier
To quantitatively evaluate the impact of microbial immigration from an upstream community
on the microbial assembly of a downstream community, an ecological genomics …