Optical Methods to Study Protein-DNA Interactions in Vitro and in Living Cells at the Single-Molecule Level

C Monico, M Capitanio, G Belcastro, F Vanzi… - International journal of …, 2013 - mdpi.com
The maintenance of intact genetic information, as well as the deployment of transcription for
specific sets of genes, critically rely on a family of proteins interacting with DNA and …

Dependence of DNA persistence length on ionic strength of solutions with monovalent and divalent salts: a joint theory–experiment study

A Brunet, C Tardin, L Salomé, P Rousseau… - Biophysical …, 2016 - cell.com
Chemistry, University of Illinois at Chicago, Chicago, IL, USA. The evaluation of nucleotide
photoionization threshold energies provides useful information for understanding …

Dependence of DNA persistence length on ionic strength and ion type

S Guilbaud, L Salomé, N Destainville, M Manghi… - Physical review …, 2019 - APS
Even though the persistence length LP of double-stranded DNA plays a pivotal role in cell
biology and nanotechnologies, its dependence on ionic strength I lacks a consensual …

High-throughput single-molecule analysis of DNA–protein interactions by tethered particle motion

T Plenat, C Tardin, P Rousseau… - Nucleic acids …, 2012 - academic.oup.com
Tethered particle motion (TPM) monitors the variations in the effective length of a single DNA
molecule by tracking the Brownian motion of a bead tethered to a support by the DNA …

How does temperature impact the conformation of single DNA molecules below melting temperature?

A Brunet, L Salomé, P Rousseau… - Nucleic acids …, 2018 - academic.oup.com
The double stranded DNA molecule undergoes drastic structural changes during biological
processes such as transcription during which it opens locally under the action of RNA …

Single-molecule tethered particle motion: stepwise analyses of site-specific DNA recombination

HF Fan, CH Ma, M Jayaram - Micromachines, 2018 - mdpi.com
Tethered particle motion/microscopy (TPM) is a biophysical tool used to analyze changes in
the effective length of a polymer, tethered at one end, under changing conditions. The tether …

Enhanced tethered-particle motion analysis reveals viscous effects

S Kumar, C Manzo, C Zurla, S Ucuncuoglu, L Finzi… - Biophysical journal, 2014 - cell.com
Tethered-particle motion experiments do not require expensive or technically complex
hardware, and increasing numbers of researchers are adopting this methodology to …

Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP

E Crozat, C Tardin, M Salhi, P Rousseau… - Nature …, 2020 - nature.com
The ter region of the bacterial chromosome, where replication terminates, is the last to be
segregated before cell division in Escherichia coli. Delayed segregation is controlled by the …

Real-Time Single Molecule Tethered Particle Motion Experiments Reveal the Kinetics and Mechanisms of Cre-Mediated Site Specific Recombination

HF Fan - Biophysical Journal, 2012 - cell.com
Tyrosine family recombinases (YRs) are widely utilized in genome engineering systems
since they can easily direct DNA rearrangement. Cre recombinases, one of the most …

Electric-field dependent conformations of single DNA molecules on a model biosensor surface

EA Josephs, T Ye - Nano letters, 2012 - ACS Publications
Despite the variety of nucleic acid sensors developed, we still do not have definite answers
to some questions that are important to the molecular binding and, ultimately, the sensitivity …