To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Abstract Alzheimer's disease (AD), the most common form of dementia, is characterized by
progressive neurodegeneration leading to severe cognitive decline and eventual death. AD …

Direct lineage reprogramming involves the conversion of cellular identity. Single-cell
technologies are useful for deconstructing the considerable heterogeneity that emerges …

INTRODUCTION Fluctuations have long been known to dynamically shape microstate
distributions in physical systems. Throughout engineering,“dithering” approaches that …

The mechanoreceptive sensory hair cells in the inner ear are selectively vulnerable to
numerous genetic and environmental insults. In mammals, hair cells lack regenerative …

Cell-fate conversion generally requires reprogramming effectors to both introduce fate
programs of the target cell type and erase the identity of starting cell population. Here, we …

Regulation of global transcription output is important for normal development and disease,
but little is known about the mechanisms involved. DNA topoisomerase I (TOP1) is an …

Cellular identity is established through complex layers of genetic regulation, forged over a
developmental lifetime. An expanding molecular toolbox is allowing us to manipulate these …

Type 1 conventional dendritic cells (cDC1s) are rare immune cells critical for the induction of
antigen-specific cytotoxic CD8+ T cells, although the genetic program driving human cDC1 …

Within mammalian systems, there exists enormous opportunity to use synthetic gene circuits
to enhance phenotype-based drug discovery, to map the molecular origins of disease, and …